• Cup type ultrasonic cell crusher
  • Lawson98-III
  • Cup type ultrasonic cell crusher
  • Shattered capacity (0.1-2ml) × 4, power 1200W.
Non-contact cup-type ultrasonic cell disruptor Lawson98-III is used for aseptic disruption and can break chromosomes and crush cells through centrifuge tubes. It comes with a silencing and pressure-tightening device and can be optionally equipped with a DL-1510 low-temperature cooling liquid circulating machine.
Product Introduction
Lawson98-III non-contact cup-type ultrasonic cell disruptor is used for aseptic disruption and can break chromosomes and crush cells through centrifuge tubes. It is suitable for ChP, ChP-seq, RNA-seq, chromatin shearing, and DNA shearing (used for second-generation sequencing). It is tailored for pre-processing DNA and chromatin immunoprecipitation experimental samples for second-generation sequencing. Compared with traditional probe-contact ultrasonic cell crushers, the non-contact sample can be disrupted under a sealed container without generating infectious aerosols. The ultrasonic probe does not come into contact with the sample, avoiding cross-contamination. Non-contact ultrasonic cell crushers can achieve unparalleled quality, efficiency, and safety compared to traditional ultrasound methods.
Multiple samples can be tested simultaneously, with high experimental efficiency and no wear and tear or debris. Each sample is in a separate fully enclosed test tube to avoid cross-contamination. It can be optionally equipped with a cooling water circulation system, which is convenient for sample ultrasound at 4°C, ensuring even energy distribution and complete ultrasonic action. The ultrasonic parameters can be flexibly set, and the experimental steps are standardized, with good experimental reproducibility and high result reliability.
It is gradually becoming an essential standardized tool for ChIP (chromatin immunoprecipitation) and DNA shearing research platforms. With high experimental efficiency, reliable results, and good reproducibility, it can process samples as low as 5ul and is suitable for precious samples.


Working Principle:
Non-contact cup-type ultrasonic cell disrupter adopts a design where the ultrasonic generator is installed at the bottom of the water tank. In traditional probe-type ultrasonic disrupters, the microfluidic phenomenon caused by ultrasound can only occur in the area close to the probe. However, the non-contact cup-type ultrasonic cell disrupter has the ultrasonic generator installed at the bottom of the water tank, which puts the entire tank within the range of ultrasonic waves. The ultrasonic effect is widely and evenly distributed, reducing the formation of foam. During the experiment, the non-contact cup-type ultrasonic cell disrupter automatically rotates the centrifuge tube to evenly distribute the ultrasonic energy. In the experimental process, each sample is placed in a separate fully sealed centrifuge tube, and there is no cross-contamination between the samples, avoiding the spread of aerosols.


Advantages:
Traditional probe-type ultrasonic cell disruptors directly contact the sample, causing metal ion contamination and only allowing for the processing of one sample at a time, resulting in long experimental cycles. For multiple samples, the same probe needs to be repeatedly used, leading to potential sample cross-contamination. Since the depth of each probe insertion and the energy distribution of each ultrasonic wave is different, the repeatability and accuracy of experimental results are affected. In addition, due to the inability to use a closed system, the aerosols or foam generated during the ultrasonic process may spread to the environment, resulting in potential biological hazards. In contrast, the non-contact cup-type ultrasonic cell disruptor can simultaneously process 4-32 samples, improving experimental efficiency. There is no need for frequent probe operations, and each sample is contained in a separate fully sealed tube, avoiding cross-contamination. The use of a 4°C water bath ensures even distribution of ultrasonic energy and complete ultrasonic effects. The ultrasonic parameters can be flexibly adjusted, and the experimental steps are standardized, resulting in good experimental repeatability and high reliability of results.


Product Features
⒈No aerosol or floating particles produced, enhancing biosafety (e.g. for bacteria, viruses, etc.)
⒉Eliminates the risk of cross-contamination between samples
⒊Eliminates the problem of probe wear and tear in traditional methods
⒋Capable of processing a wide range of samples
⒌Suitable for use with various standard containers
⒍Can process tiny sample volumes, as low as 5μl
⒎Automatic continuous rotation of centrifuge tubes ensures even distribution of ultrasonic energy
⒏Optional high and low-temperature constant temperature water bath, and customized rotation base for Eppendorf tubes of various diameters, based on customer requirements


Application Scope
⒈DNA fragmentation for second-generation sequencing samples
⒉RNA fragmentation
⒊Bacterial and cell lysis
⒋ChIP assay (Chromatin Immunoprecipitation)
⒌Preparation of samples for high-throughput sequencers
⒍Extraction of membrane proteins/proteins
⒎Homogenization, emulsification reactions
⒏Ultrasound treatment of valuable reagents


Product Advantages
Closed system prevents potential biological hazards caused by the diffusion of aerosols or foams generated during ultrasonic processing. The contactless fully automatic ultrasonic disruptor can detect 4-32 samples at the same time, with high experimental efficiency. There is no need to frequently operate the probe, and each sample is in a separate fully enclosed test tube to avoid cross-contamination. The ultrasonic energy distribution is even and the ultrasonic action is complete. The ultrasonic parameters can be flexibly set, and the experimental steps are standardized, with good experimental repeatability and high result reliability.


Technical Specifications  
Model: Lawson98-III
Frequency: 19.5-20.5KHz
Power: 1200W
Power Adjustment Range: 450-1200W continuously adjustable
Time Control Precision: ±1% arbitrary setting
Working Cycle Setting: ±1% arbitrary setting
Ultrasonic Time Setting: 1-99 cycles, digital display
Interval Time Setting: 1-99 cycles, digital display
Horn End Diameter: Φ30
Crushing Capacity: (0.1-2ml)×4
Duty Ratio: 1-99%
Power Supply: 220/110V 50Hz/60Hz
Working Environment: 0-32℃, RH80, 760±30mmHg
Transducer assembly weight: 4.5kg
Transducer weight: 6kg
Generator dimensions: 140×330×210mm


Precautions
1. Choose an appropriate probe according to the volume of the sample being processed. The probe must be replaced with a dedicated wrench;
2. When the AMPLITUDE knob is turned on, the probe must not be exposed to air. In special circumstances (testing probe), it should not exceed 10 seconds;
3. Prepare an ice box before use, and the sample must be placed in an ice bath during sample processing. The sample concentration should not be too high.
 
 


Single Page - Color Page    
Name: Author: Action:
Cup type ultrasonic cell crusher    Lawson98-III ultrasonic Download

 

 

 

 

Product List
Lawson98-III Unit
Host Machine Unit
Adapters set
Special Cooling Equipment Set
Power cord piece
User manual copy
Warranty card copy
Certificate of qualification copy
Description
1
Choose any one
Optional
1
1
1
1